FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n01_reseq-total_drought_1h_rep2_shoot.35100000073a56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n01_reseq-total_drought_1h_rep2_shoot.35100000073a56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216845
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT105114.847241116926837No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC38031.7537872674029837No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG34211.5776245705457814No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT28621.319836749752127No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA15470.7134128063824391No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC14050.6479282436763587No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC12540.5782932509396113No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT8830.40720330189766885No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT8400.38737346952892615No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG8120.37446102054462865No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT8010.3693882727293689No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA7580.3495584403606262No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC6660.3071318222693629No Hit
CCACAGCCTAATCCTTATTTTATTCCTACAAATAGAACATAGCCATACGA6480.29883096220802874No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT5960.27485069980861904No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG5570.2568655030090618No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA5140.23703567064031913No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG4750.21905047384076182No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC4620.2130554082409094No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCATAACATCCTTAT4530.20890497821024234No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT3480.16048329451912655No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT3390.1563328644884595No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG3330.15356591113468146No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA3330.15356591113468146No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC3210.14803200442712536No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT3200.14757084553482902No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA3050.1406534621503839No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA2980.13742534990430952No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG2940.13558071433512417No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT2930.1351195554428278No Hit
GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG2900.13373607876593882No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA2880.13281376098134612No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA2800.1291244898429754No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT2680.1235905831354193No Hit
GTATTGCAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGTAATGCACC2590.11944015310475224No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA2460.1134450875048998No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC2450.11298392861260347No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT2450.11298392861260347No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG2410.11113929304341812No Hit
GATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATAT2390.11021697525882544No Hit
CTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGC2390.11021697525882544No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA2390.11021697525882544No Hit
GCTAATGAGGGTTACAGATTTGGTCAAGAGGAAGAAACTTATAATATTGT2370.10929465747423275No Hit
ATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCT2340.10791118079734373No Hit
ACCACCAGAGCACAAGAAAGCATAAGCAACACAAGGGATTAACTCATCAC2320.10698886301275104No Hit
CAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGC2280.10514422744356568No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC2250.10376075076667665No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG2230.10283843298208398TruSeq Adapter, Index 11 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC14000.072.93971
AGCCTTC14100.072.405695
AAGCCTT14050.072.342084
CCTTCTA14600.070.251287
CTTCTAT14700.069.450368
GCCTTCT15150.068.0143366
TCCTAGT407.539338E-659.355838
GTGACCC407.539338E-659.355836
CCCGTAA250.005017627556.994741
ACAGCCT1000.056.994743
TTCTATT18150.056.249059
CGGGTAG602.3412213E-855.411551
CCTAGTC352.78733E-454.2681859
CACAGCC1000.052.245182
CAGCCTA1100.051.81344
AGCCTAA1100.051.801455
GGTAGGT707.886774E-847.4956173
CGAGTCG205.3930253E-447.48466530-31
CTATCGG405.387023E-447.4846657
GTAGACC804.2091415E-947.4846656
Produced by FastQC (version 0.11.2)