FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l01n02_reseq-total_salt24h_rep1_shoot.352000000739a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l01n02_reseq-total_salt24h_rep1_shoot.352000000739a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70419044
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG8263151.1734254728024993No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6798300.9654064602183466No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4475310.6355255263050716No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT4429920.6290798267582275No Hit
GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG4029210.5721761857488438No Hit
GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG3695510.5247884364916968No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3695390.5247713956468935No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3485540.49497121829714136No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3306760.46958319968104084No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3268400.46413580962558937No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3048780.4329482234947694No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3002580.42638749824550304No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2951400.41911957793690013No Hit
GTCCCCATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG2346910.3332777423107306No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2132140.3027788903240436No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG2076140.2948264960825086No Hit
CCCCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCC2054770.2917918056371228No Hit
AGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTA2008070.2851600768678427No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1959470.27825853472251055No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1892530.2687525834630757No Hit
CTCGCCTGTATTTAGCCTTGGACGGAGTTTACCGCCCGATTTGGGCTGCA1863820.26467556134388875No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1852190.26302401946836995No Hit
CCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATC1491760.21184042203129028No Hit
ATGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTTGCTACGCCCTTCC1461510.2075447090704611No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1359040.19299324767885231No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1341690.19052942553437674No Hit
GTCCCAATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG1335180.1896049597037983No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1283640.18228591686078555No Hit
GTCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG1281760.18201894362553403No Hit
ATCCCTATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG1260470.17899561374335046No Hit
CGCCTGTATTTAGCCTTGGACGGAGTTTACCGCCCGATTTGGGCTGCATT1212770.17222187793404295No Hit
CGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG1195930.16983047937998136No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1193710.1695152237511205No Hit
CCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT1077500.1530125856295351No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1076060.1528080954918956No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1053920.14966405962568877No Hit
CCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTCGGGGGCG1032440.14661374840589997No Hit
GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA1011960.1437054442261386No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA996870.14156255799212497No Hit
GTCCCGATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG990560.14066649356955202No Hit
GGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTA989480.1405131259663224No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA950140.13492656901164407No Hit
CGTTGGATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCA940770.13359596304658722No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA909870.12920794550974024No Hit
ACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACG901070.12795828355749903No Hit
ATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGA894900.1270821001205299No Hit
CCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCA888680.1261988163315594No Hit
GCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTT876110.12441378783841484No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA862120.12242710934843137No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT856510.12163044985387758No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA851260.12088491289373368No Hit
GTCCTTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG796340.1130858862554283No Hit
GGCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG787610.111846164795989No Hit
GTTCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG777530.1104147338325127No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG769920.10933406025790411No Hit
CCCGTCACCACCATGGTAGGCCCCTATCCTACCATCGAAAGTTGATAGGG767870.1090429458258479No Hit
GTCGAGTTATCATGAATCATCGGATCAGCGGGCGGAGCCCGCGTCAGCCT759420.10784298633761627No Hit
GTCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGA752190.10681627543821809No Hit
ATCCCGATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG746020.10594009200124899No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT738410.10485941842664039No Hit
GTTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCG731030.1038114064712381No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA709120.10070003222423753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGA2094700.079.164497
CACGATG2130550.077.994869
TCACGAT2142450.077.4294748
TCCCCAT1817550.077.154312
CATCACG1814600.077.078816
GAATTGC1256700.075.560396
ATTGCAG6064800.075.431668
TTGCAGT6061750.075.308319
CTATAAT560850.075.154613
CTCTACA473350.075.12461
GTCCCCA582900.074.291591
ATCCCCA1250500.074.083981
TAATTGC777600.073.1701056
CCCCATC1649350.072.898863
CCGCGGA509850.072.321952
TCTATAA569800.071.818762
TATCACG236100.070.831246
CCATCAC1871550.070.386675
CTACAAT520550.070.2374043
CCCATCA1835200.069.4361954
Produced by FastQC (version 0.11.2)