FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l01n02_reseq-total_ctrl_1h_rep3_shoot.35200000073ad9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l01n02_reseq-total_ctrl_1h_rep3_shoot.35200000073ad9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90815686
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG20912542.302745364936185No Hit
GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG5792180.6377951051319483No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4976130.5479372803504452No Hit
GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG4864220.5356145192802926No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT4106950.4522291446435806No Hit
CTCGCCTGTATTTAGCCTTGGACGGAGTTTACCGCCCGATTTGGGCTGCA4059990.44705823176846343No Hit
GTCCCCATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG3424590.37709234503827893No Hit
CAATGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTC3356760.36962337101103876No Hit
CCCCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCC3219250.35448171365462133No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3201210.352495272677894No Hit
ACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACG2952820.32514427078159164No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2943450.3241125106955642No Hit
CGCCTGTATTTAGCCTTGGACGGAGTTTACCGCCCGATTTGGGCTGCATT2911980.32064725029990965No Hit
GTCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG2885250.3177039261697588No Hit
CCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTCGGGGGCG2813820.30983854485226264No Hit
ATGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTTGCTACGCCCTTCC2791270.3073554936313535No Hit
TCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTG2615290.2879777839260059No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2554940.28133245615740876No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2521030.2775985197094696No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2511870.2765898833820404No Hit
AGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTA2455480.27038060363272487No Hit
GGCCCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAGGCCTACGC2332200.2568058562041804No Hit
CCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGG2328080.2563521900831096No Hit
CCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATC2324470.2559546816614918No Hit
TGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTC2202290.24250105868274782No Hit
CCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCA2175360.23953571192536055No Hit
ATCCCTATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG2098690.23109333777426955No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2085060.22959249572810583No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2025420.22302534828619805No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1941300.21376263127054945No Hit
GTCCCAATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG1880830.2071040899256104No Hit
TCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTCGGGGGCGGCG1800440.1982520949079215No Hit
GCCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACG1754740.19321992458439394No Hit
GTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCC1731210.19062896249002623No Hit
TCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTCGGGG1719650.18935605463575972No Hit
GTCCCGATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG1665090.18334828192565764No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1580290.17401068797740513No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1579530.17392700199390665No Hit
TCCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACG1527620.16821102909468746No Hit
CCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGG1505380.16576211294599483No Hit
AAGCTGCGCAGGAAAGGCCCAAAGCCAATCCCAGGGAACAGTAAAGCTTC1492120.16430201276021852No Hit
CCTTCCTCCGGCTTAACACCGGCGGTCTGTTCAGGGTTCCAAACTCATAG1475450.16246642678006087No Hit
ATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGA1470590.16193127693821527No Hit
CCCGTCACCACCATGGTAGGCCCCTATCCTACCATCGAAAGTTGATAGGG1438200.15836471245727307No Hit
TGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACT1416320.15595543703760603No Hit
CTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATC1404080.15460765225073564No Hit
GGCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG1397300.15386108518742014No Hit
GCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGA1391970.1532741821715689No Hit
GGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTA1320710.1454275200872237No Hit
GCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCC1318290.14516104629766272No Hit
GCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGAAACCTTTGCA1305780.1437835309640231No Hit
ATGCTCATTTTCAACTCCCCGAAGCATTTCGTCGCTTGCTACGCCCTTCC1261290.138884597535276No Hit
CTCGCTTGTATTTAGCCTTGGACGGAGTTTACCGCCCGATTTGGGCTGCA1259620.13870070859785172No Hit
GTTCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG1255340.13822942437499178No Hit
GTCCTTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG1253530.13803011959850198No Hit
TTCCCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTT1224480.13483133299240838No Hit
CTCGCGTGTATTTAGCCTTGGACGGAGTTTACCGCCCGATTTGGGCTGCA1182940.13025723331539885No Hit
GTCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGA1156080.12729959447754433No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1134220.12489252132060094No Hit
GTTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCG1119070.12322430730744025No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1112990.12255481943945234No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1107330.12193157908866095No Hit
GGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCG1105030.12167831887544185No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1084360.11940228035055529No Hit
CAATGTATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTC1076520.11853899336288667No Hit
TGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCG1074190.11828242975558209No Hit
ATCCCGATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG1070180.11784087607949137No Hit
CGATGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTC1058920.11660100216607955No Hit
CCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT1053740.11603061612065564No Hit
CAGTGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTC1035850.11406069211435566No Hit
CATGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTCC1021860.11252020933916636No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1012320.11146973001998794No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1009890.11120215509906514No Hit
GCCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG1007000.11088392813549852No Hit
ATCCTCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG990800.10910009532934652No Hit
TTTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGA983310.10827534793934168No Hit
GTCGAGTTATCATGAATCATCGGATCAGCGGGCGGAGCCCGCGTCAGCCT967540.10653886378174801No Hit
CGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG962790.10601582638488245No Hit
TCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACT962690.10600481507126423No Hit
CCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGGA962570.10599160149492237No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA961860.10591342116823299No Hit
CCCCGCTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGG959300.10563153153960649No Hit
TCCACCTAAGCTGCGCAGGAAAGGCCCAAAGCCAATCCCAGGGAACAGTA947320.10431237616814347No Hit
GCCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCA944740.10402828427679334No Hit
CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT941160.10363407924926096No Hit
TAATGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTC937290.10320794141223577No Hit
CCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTTGA937280.10320684028087394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCA2579750.087.694461
TCCCCAT3494950.086.3891752
ATCACGA4210800.085.9772957
CATCACG3771200.085.176816
CACGATG4291250.084.4189
TCACGAT4296150.084.235178
GTCCCCA833650.083.971711
CCATCAC3635350.083.526285
CCCATCA3560950.083.026874
TATCACG337500.082.194336
CTCTACA277900.079.906621
TAATTGC406400.079.715756
GAATTGC728950.079.1091166
ATTGCAG3714000.079.090758
GGTCCCT371000.076.3463061
CTATAAT356650.076.2135853
CTATCAC364700.075.884695
CGATTAA259350.075.579895
TTGCAGT3879000.075.5633549
TCGCGTG179900.075.049512
Produced by FastQC (version 0.11.2)