FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l01n02_reseq-total_ctrl_1h_rep2_shoot.35200000073a6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l01n02_reseq-total_ctrl_1h_rep2_shoot.35200000073a6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63417262
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA9649631.5216093687551506No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG6326350.9975753920123515No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6072450.9575389741676328No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5364270.8458690632213041No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA5346120.8430070664356338No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4810210.7585016836583075No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4620030.7285130032892306No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4430240.6985858203717468No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4399280.6937038688299094No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT3949120.6227200411143577No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3761800.5931823420569624No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3118340.49171785435959064No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2757130.43476017618042223No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2459440.3878186983222328No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA2331160.3675907673213643No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG2330260.3674488501253807No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2316750.3653185153278929No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT2283180.36002500391770303No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT1963810.30966489849404094No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1890090.29804030328524744No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1812230.28576288897492924No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1618160.25516081094765647No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1576150.24853643161068673Illumina Single End PCR Primer 1 (100% over 50bp)
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1481980.23368716233759823No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1405780.22167150641098318No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1324480.20885165304046083No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1307640.20619622461783357No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1207440.1903961101316547No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1190580.18773752799356114No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1189870.18762557109450734No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1161970.18322613801901444No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1150710.18145059621148577No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA965780.15228976615231354No Hit
CTCTCGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA958920.15120804174737157No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA950140.14982356065766447No Hit
CTCTTGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA947610.14942461565117712No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG859340.13550569244064808No Hit
TGACCTAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT850310.13408178990761221No Hit
CTCTGCAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA831210.1310699916372927No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT827440.13047551627189455No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG825730.13020587359952562No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT822510.12969812540945083No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG810390.1277869738368711No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT806050.12710261758068334No Hit
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG777120.12254076815867579No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG746940.11778181152002432No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT732510.1155064058110866No Hit
TCAAGAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA706130.11134665511103271No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT704840.11114324046345614No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG680750.10734459018429399No Hit
GCCGTTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG678750.10702921863766365No Hit
CTTTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA667880.1053151742817279No Hit
CTCCGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA662370.10444632567076137No Hit
GTCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA656890.1035822076329943No Hit
CTCTGTAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA636000.10028815182844064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA341700.079.2747961
CCGCGGA844300.078.036162
CTCTACA662650.077.8687741
GCCGCGG764300.076.686991
CTCTATA768000.075.766621
CGGAAGT1222000.075.01915
CTATAAT818100.074.715893
TTGCAGT9086050.074.3970959
GAATTGC1909900.074.3442466
ATTGCAG9147200.074.0505458
TAATTGC1178200.073.928836
CGCGGAA1054250.073.710763
TAGAATT1037750.073.6324
CGAATCT500700.073.522745
GACCGAA376450.073.257052
CTACAAT736000.073.113763
CTAGAAT972200.072.678053
CTCTAGA928200.071.386591
GCGGAAG1259000.070.947354
ACCGAAT408150.070.753273
Produced by FastQC (version 0.11.2)