FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l01n01_reseq-total_salt24h_rep3_shoot.351000000739f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l01n01_reseq-total_salt24h_rep3_shoot.351000000739f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63271452
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT18914152.9893655672703705No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG9181821.4511789614058486No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC3431630.5423662475771853No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA2797300.44211092231611815No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT2567500.4057912247691108No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT2443850.3862484458235604No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC2095210.3311461858027219No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA2028270.3205663748636589No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT1689730.2670604113842685No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1646280.26019317527279123No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT1645930.26013785806590944No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1632740.2580531896122757No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1614540.25517669485441874No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC1496580.23653321564360494No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1487990.23517557333756148No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC1412140.2231875443604487No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC1370220.21656212346762646No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA1190820.18820810371160757No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA1052330.1663198751942661No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC953640.15072200334520536No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA938290.14829594870052926No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG928570.1467597108408386No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG907370.14340906859542277TruSeq Adapter, Index 5 (100% over 49bp)
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA848680.13413316324714658No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT810650.12812255359652566No Hit
CTGGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCT799850.1264156226413138No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG793680.1254404593085678No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT775520.12257028651721158No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC758860.1199371874696348No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA711950.11252310125583968No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCATAACATCCTTAT709950.11220700293080045No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT674840.10665789683473678No Hit
CCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACT652220.1030828247785431No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT648360.10247275501121737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC3042550.065.542491
AGCCTTC3202900.063.1279145
AAGCCTT3189550.063.094344
CCTTCTA3278900.062.1295557
CTTCTAT3378250.060.465438
GCCTTCT3488300.058.4891476
TTCTATT3816100.053.543999
ACAAGCC5358050.037.8226052
CAAGCCT5438750.037.479643
GGTGTGC512600.035.742457
CGGGTAG183300.035.638451
TGTGCTC563500.035.4038629
TCTATTA3443650.035.04253410-11
TTATCTT3620300.033.35085314-15
ATCTTTC3628050.033.13767216-17
CTATTAT3348350.033.07356310-11
CTTTCTA3686200.032.8112518-19
GGGTAGG213650.030.5564612
TTTCTAG3616500.030.4633818-19
TATCTTT3708150.029.88916814-15
Produced by FastQC (version 0.11.2)