FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l01n01_reseq-total_salt24h_rep1_shoot.351000000739a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l01n01_reseq-total_salt24h_rep1_shoot.351000000739a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70419044
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT22850833.2449787304695588No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG14988112.1284171367052354No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA8343561.1848442588911035No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTG8150631.157446840658615No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGA4409230.6261417011000604No Hit
CAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGTACCCAGAGACGAGGAA3849870.5467086431903279No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC3319710.47142219084939585No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT2651810.3765756888150881No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC2637510.3745449881426962No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA2610430.3706994374987539No Hit
CCAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACT2380860.3380988813196612No Hit
CAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTGCTTATAGGACT2308490.32782183183287744No Hit
AAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCG2303880.3271671793783511No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG2241830.31835564254465026No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTC2179720.3095355852885478No Hit
AACACGGACCAAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAAC2149110.3051887497933088No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC2090060.2968032340796901No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT2086740.29633177070679917No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA2022430.2871992979626363No Hit
GCCCAGCTTGAGAATCGGGCGGCCGCGCCGTCCGAATTGTAGTCTGGAGA1998910.2838592923811917No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAA1879170.2668553694083095No Hit
GACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCGCTTTAGTGTC1752250.2488318358880305No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1742130.24739472464295312No Hit
AGCATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGG1741270.24727259858852957No Hit
TAGCATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGG1724280.24485989897846386No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA1669080.23702111036895077No Hit
GCAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACT1655630.23511111568058207No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1648590.23411138611878912No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1570480.22301921622224805No Hit
TAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAG1537410.2183230434085416No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1536550.218200917354118No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC1469540.20868502560188124No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT1409610.20017454369303847No Hit
ACAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACT1344210.19088728327524582No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT1335930.18971146498381886No Hit
GGATACATTAGCATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTG1300390.1846645347812447No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAC1045190.14842433816624948No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGT1013940.14398661816539288No Hit
ATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT1013320.14389857380057589No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC1006730.14296274740679524No Hit
TATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGTCGGG947720.13458291197477773No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC945820.13431309859872564No Hit
AGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAA905460.12858169446321935No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT902870.12821389622954837No Hit
CATGGAGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAAC892160.1266930008308548No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA871460.12375345510228739No Hit
ACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTT857850.12182073928751433No Hit
CGCCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCATCGCTCTGG850490.12077556747291258No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA837900.11898769883896748No Hit
CAAAGCAAGCCATCGCTCTGGATACATTAGCATGGGATAACATCATAGGA833780.11840262983405456No Hit
CTGGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCT830340.11791412561636026No Hit
CAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTA816740.11598282987198746No Hit
TAATAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTC812550.11538782037427261No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG790100.11219976232565725No Hit
TGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGAC789050.11205065493362847No Hit
AGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTT787730.11186320564079229No Hit
AGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGG759250.10781884514081162No Hit
AAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT757010.10750074937115023No Hit
CATAGTCAAAATCTGAGTTTGATCCTGGCTCAGAAGGAACGCTAGCTATA744350.1057029402444032No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA740880.1052101758155081No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA714140.10141290756517513No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC710870.10094854454428549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC3377500.070.585541
AAGCCTT3637600.066.233054
AGCCTTC3694050.065.557635
CCTTCTA3782200.064.574977
CTTCTAT3852350.063.574088
GCCTTCT3922800.062.3491176
ACCTGGT1513150.058.6841961
TTCTATT4198100.058.339429
CCTGGTT1579100.056.9176452
CTGGTTG1635050.054.6824873
GTTGATC1651400.054.4719056
TACCTGG523000.053.2801171
GGTTGAT1694500.052.9130975
CAACACG2467950.051.9748341
CACGGAC462700.051.676393
CGGACCA466800.051.423785
TGGTTGA1775700.050.328694
CCAACAC729050.050.061811
TGATCCT1808650.049.8968058
ACAACAC430850.049.0879021
Produced by FastQC (version 0.11.2)