FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l01n01_reseq-total_ctrl_1h_rep3_shoot.35100000073adc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l01n01_reseq-total_ctrl_1h_rep3_shoot.35100000073adc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90815686
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT19885522.1896569718143186No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGA11969811.3180333186053343No Hit
CAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGTACCCAGAGACGAGGAA10667491.1746307790925017No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA10622701.1696988117229No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTG9130951.0054375408230687No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTC8811140.9702222587406322No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG7680520.8457261447102871No Hit
TAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAG6897440.759498750028712No Hit
CAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTGCTTATAGGACT6207970.6835790460251547No Hit
GGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAA5909650.6507300952392739No Hit
GAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAG5839130.6429649168757036No Hit
AACACGGACCAAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAAC4942650.5442506925510644No Hit
GGGCACTGTCTCGGAGAGAGACTCGGTGAAATAGACATGTCTGTGAAGAT4873130.5365956273236762No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGC4704620.5180404627456098No Hit
TGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTCCCCTGG4422160.4869379062995791No Hit
CATGGAGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAAC4357160.47978055244773465No Hit
ACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATT4130830.4548586463356121No Hit
GCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTT4082970.44958863163793095No Hit
GCCCAGCTTGAGAATCGGGCGGCCGCGCCGTCCGAATTGTAGTCTGGAGA3974190.43761052468402867No Hit
AAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGT3901780.4296372324930739No Hit
GACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCGCTTTAGTGTC3845420.4234312561378438No Hit
GTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTCCCCTGGA3831400.4218874699685691No Hit
TATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGTCGGG3827840.42149546720376035No Hit
CCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTGAGAGACGAAA3763450.414405282364987No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT3688050.4061027518968474No Hit
GCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAA3450900.3799894216512333No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC3375460.37168248665764636No Hit
ACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGA3246660.357499914717376No Hit
AAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCG3176630.34978869179053496No Hit
GCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGACTCGGTGA3059990.3369450955862405No Hit
GGGCGGAAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGT2936850.32338576399676155No Hit
GCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGA2796350.30791486836315923No Hit
CAAAGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAA2454110.2702297486361552No Hit
CCAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACT2408830.2652438258298242No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC2274360.2504369124074006No Hit
TAGCATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGG2230810.24564148532666483No Hit
GTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGAC2199310.24217292153692482No Hit
GACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCAGT2035280.22411106380895474No Hit
GGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAG1947910.21449047910071395No Hit
GAAACATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCGTAGT1908750.21017844868781807No Hit
GCAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACT1791420.1972588744195579No Hit
CCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGG1788380.1969241304855639No Hit
AGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGG1786280.19669289289958122No Hit
ACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTT1773310.1952647255232978No Hit
CAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTA1766150.1944763154682331No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA1732530.19077431182978674No Hit
TGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGG1689260.18600971642718198No Hit
CAGGCAAGAGACAACCTGGCGAACTGAAACATCTTAGTAGCCAGAGGAAA1688280.1859018055537234No Hit
GTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTAT1682850.18530389122425392No Hit
GTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCGGAATGAT1674310.18436352504125772No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC1639560.1805370935589255No Hit
ATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT1591170.17520871889906772No Hit
AGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTT1582880.1742958810001171No Hit
AGCATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGG1555080.1712347358142513No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT1533380.16884528075909708No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATCCAACGAGTATA1473580.16226051521540014No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC1440260.15859154551780844No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG1440050.15856842175921018No Hit
CCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACC1413470.1556416145994867No Hit
AGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAA1400540.15421785174865058No Hit
AAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTGC1388400.15288107827539837No Hit
TGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGAC1385170.15252541284552978No Hit
CAACCTTTTGAACTGCCTGCTGAATCCATGAGCAGGCAAGAGACAACCTG1372710.1511534031686993No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1372680.15115009977461383No Hit
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAG1362400.15001813673466058No Hit
AGCAGGCAAGAGACAACCTGGCGAACTGAAACATCTTAGTAGCCAGAGGA1356180.14933323302760715No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA1352060.1488795669065364No Hit
ACAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACT1313620.14464681795169174No Hit
AGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTA1302670.1434410791104964No Hit
GGATACATTAGCATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTG1285830.14158677389718774No Hit
AAGACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTG1261210.13887578848438142No Hit
AGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCCACACCAGGCCATCT1254790.13816886215009155No Hit
GCCCTTGGGAGGGGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGG1244650.1370523149492038No Hit
TGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTT1192190.1312757798250844No Hit
ATGCCGCGTGGAGGTGGAAGGCCCACGGGTCGTCAACTTCTTTTCTCGGA1181640.13011408623836196No Hit
GCTCGACCCTTCTGCCGGCGATGCGCTCCTGGCCTTAACTGGCCGGGTCG1157430.12744824721139034No Hit
GACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAAT1156100.12730179674026795No Hit
CAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGC1151710.126818400072428No Hit
CAAAGCAAGCCATCGCTCTGGATACATTAGCATGGGATAACATCATAGGA1125790.1239642675825848No Hit
AACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAAT1094180.12048359134786474No Hit
TGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTT1094010.12046487211471374No Hit
GCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACG1067410.11753586269226662No Hit
CGCCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCATCGCTCTGG1065570.11733325452169133No Hit
TACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTG1046380.11522018343835447No Hit
AGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATG1038010.11429853648850927No Hit
AGGCCCACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTA1036690.11415318714874872No Hit
ACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGG1031530.11358500336604846No Hit
TACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAAT1025040.11287036911222584No Hit
CCAAAGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAA1021990.11253452404687006No Hit
ACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAG1019960.11231099438042014No Hit
GGGAGATGCCCAGCTTGAGAATCGGGCGGCCGCGCCGTCCGAATTGTAGT998180.10991273027437132No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG996160.10969030173928324No Hit
GCCGAAGCTGTGGGATGTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTT995950.10966717798068498No Hit
CAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTC993490.10939629966567671No Hit
TTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGA992940.10933573744077646No Hit
TGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGA959520.10565575642956658No Hit
AGGCAAGAGACAACCTGGCGAACTGAAACATCTTAGTAGCCAGAGGAAAA958090.10549829464482602No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT947930.10437954518121462No Hit
TGCCCAGCTTGAGAATCGGGCGGCCGCGCCGTCCGAATTGTAGTCTGGAG945820.10414720646387014No Hit
CAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCA942210.1037496980422523No Hit
AGCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAAT940760.10359003399478808No Hit
CATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAA939050.10340174053191649No Hit
CAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTGAGAGACGAAAG937030.10317931199682838No Hit
CACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAAT935660.10302845700025874No Hit
AGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTGCTGAATC934070.10285337711372902No Hit
AGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATAGATCCGGAG913480.10058614763973704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC2642650.078.704861
AGCCTTC2742400.076.706255
AAGCCTT2752200.076.07934
CCTTCTA2800700.075.698217
CTTCTAT2849550.074.630458
GCCTTCT2939800.072.22196
AGAGTTC683800.069.688286
AGACATT480300.069.0273748
GAGAGTT691200.068.489535
GGAGAGT690050.067.7312554
AAAGAGG1719850.066.817083
CCAACAC525200.066.577321
CACGGAC806350.066.0424653
ACACGGA807100.065.822262
GTTGATC1734750.065.205016
CAAAAGA1765600.065.080931
CTGGTTG1717750.065.071723
TTCTATT3269850.065.017349
ACCTGGT1723350.064.633171
AGGAAAG1800950.064.622947
Produced by FastQC (version 0.11.2)