FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l01n01_reseq-total_ctrl_1h_rep2_shoot.35100000073a62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l01n01_reseq-total_ctrl_1h_rep2_shoot.35100000073a62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63417262
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT44572167.028395517926965No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG14996062.364665317780512No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA5596700.8825199675129461No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC5486020.8650673061224246No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT3886900.6129088322986886No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT3195910.5039495397956475No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC2824790.44542919560292593No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT2801290.44172357993001965No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG2799670.4414681289772491No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA2786830.4394434436478825No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA2780230.4384027175440025No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC2748650.43342300082270974No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA2565220.40449869942351024No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT2526560.3984025674271463No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT2440630.3848526289261747No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC1990040.31380099632809755No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC1957710.3087030152768185No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT1871490.29510734790158555No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1649390.2600853376482889No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1529910.2412450414525938TruSeq Adapter, Index 6 (100% over 49bp)
AAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1525230.24050707203347885No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA1415280.22316952125747716No Hit
CTGGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCT1358750.21425554449197132No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT1292840.2038624751727692No Hit
GATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATAT1266560.19971849305004682No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA1208160.1905096438884416No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA1181140.18624897429346604No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCATAACATCCTTAT1129880.178166001553331No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC1109300.17492082833850506No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA1092610.17228905278187506No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG1089200.17175134429487038No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT1074350.16940971056114026No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG1064800.16790381142598051No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA1062500.16754113414735566No Hit
GAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1014550.1599801013168938No Hit
CCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACT1013720.1598492221250422No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT867430.13678137034676774No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT747250.11783069410975201No Hit
GCTAATGAGGGTTACAGATTTGGTCAAGAGGAAGAAACTTATAATATTGT738140.11639417671485092No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC733450.11565463043800282No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT701680.11064495341978026No Hit
ATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCT688780.10861080694401472No Hit
CTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTT675730.10655300760225189No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT656060.10345132844114273No Hit
TATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCC643890.10153229257989725No Hit
CCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTAC639810.10088893462477141No Hit
GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG636930.10043479959762375No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG636240.10032599641403629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC6299550.074.87061
AAGCCTT6826500.069.8203664
AGCCTTC6918400.069.261555
CCTTCTA7095300.068.102427
CTTCTAT7208500.067.1938
GCCTTCT7352600.065.8002246
TTCTATT8021200.060.3739249
ACAAGCC9486850.050.3526572
CAAGCCT9675050.049.6058043
TAACAAG449050.038.23951
TAGCCTG131900.037.929461
TCTATTA7467200.037.6056310-11
TTATCTT7627650.036.76645314-15
ATCTTTC7727700.036.15996616-17
CTATTAT7213050.035.8317610-11
CTTTCTA7956450.035.48552318-19
CGGGTAG182450.034.6603241
CCGAGCC166300.034.0319863
GGGTAGG189800.033.1954082
TTTCTAG7789550.033.01288218-19
Produced by FastQC (version 0.11.2)