FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n02_58.35200000068041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n02_58.35200000068041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397720
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG198150.020.273215
TAATACT231500.019.751764
CTATACT207200.019.7307644
GTATTAT217800.018.768741
TATACCG103900.018.5091195
TTATACT239000.018.118724
GTATTAG141550.017.743351
GTATAGG164950.017.6152551
ATAATAC209900.017.5992763
GTATAAT226800.017.4378011
ACTGTGC146350.017.3613328
GTATATA417750.017.0817491
ATTATAC213950.016.6003423
TAGACTG107750.016.085035
CCTATAC162550.016.0658383
GTATAAG141200.015.9043621
TATACTA277450.015.7110135
TATACAG185700.015.4955485
ACTATAC161850.015.489913
GTATAGA163200.015.3022531
Produced by FastQC (version 0.11.2)