FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n02_57.35200000067fd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n02_57.35200000067fd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19206232
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC254850.13269130561371956No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG216660.11280713468420041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT147250.020.927024
TATACTG132800.020.2017885
GTATAGG113200.019.6698171
TAATACT170250.019.4664274
TTATACT175000.019.3450324
GTATTAT152650.018.3186021
TAGACTG67400.017.964695
CCTATAC115050.017.9110033
TATACAG126400.017.3178235
GTATAAT163550.017.0977361
TATACCG63300.017.027925
ATAATAC150450.016.915683
ATTATAC154000.016.864893
ACTGTGC93850.016.7496018
GTATATA298350.016.7403131
TATACTA209050.016.7400515
GTATAAG102950.016.4632551
GTATTGG134750.016.4184061
TACACTG95900.016.3888635
GTATTAC98850.016.0414521
Produced by FastQC (version 0.11.2)