FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n02_56.35200000067f6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n02_56.35200000067f6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15426126
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG109400.021.3551185
ACTGTGC71250.019.7304538
CTATACT132900.018.5072574
CTGTGCG35500.018.1942929
TTATACT178450.017.9341434
AGACTGT48450.017.2523276
ATACTGT102500.017.1901196
TAATACT188550.017.1245694
ACTGTGT80450.016.9428358
GTATTGG96400.016.8932631
GACTGTG41450.016.8431387
TAGACTG48450.016.5633095
GGACTGT43500.016.3768676
GTATTAC84250.016.3427281
CTACACT70300.016.210384
TACACTG72600.016.0244675
TACTGTG78700.015.7505617
TAGACAG52150.015.6613195
GTATAGG89300.015.63121
TAGGACT64150.015.3958614
Produced by FastQC (version 0.11.2)