FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n02_55.35200000067ee6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n02_55.35200000067ee6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22923986
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG135100.021.120181
CTATACT167550.019.3559914
CCTATAC144950.018.4754223
TATAGGG129250.017.1193732
TATACTG131600.016.994785
TAGACTG76550.016.4380865
TATACTA221950.015.9172595
CCCTATA137500.015.6432882
GTATATA295000.015.5948171
GTATTAG98200.015.2292181
TATACCG73000.014.8957765
TATACAG127900.014.8504625
TTATACT165200.014.5438594
TACACTG106150.014.3146425
ACTGTGC101550.014.2639138
TCTATAC108350.014.2425573
TAATACT159750.013.9997154
GTATTAT151250.013.7171721
TAGACAG73700.013.4657125
GTATAAG104450.013.2725071
Produced by FastQC (version 0.11.2)