FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n02_54.35200000067e53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n02_54.35200000067e53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22097550
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG149850.024.2697181
CTATACT181050.022.1606924
CCTATAC156750.020.9918083
TATACTG139300.020.0095675
TATAGGG141350.019.8524842
TAATACT175700.018.5386244
TATACTA244350.018.2669585
TTATACT177000.018.0000784
TAGACTG78800.017.776515
GTATTAT161050.017.4523411
CCCTATA146800.017.0454222
ATAATAC158400.017.0261843
GTATATA313950.016.84681
GTATTAG103650.016.810841
TATACAG136500.016.4542925
GTATAAG106550.016.264181
ATTATAC155850.016.238453
TATACCG68950.015.8396015
GTATAAT168650.015.5679531
TCTATAC113900.015.5492433
Produced by FastQC (version 0.11.2)