FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n02_51.35200000067e02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n02_51.35200000067e02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23864018
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG140500.016.9995085
GTATATA310350.015.0529181
CTATACT143600.014.6808254
TAATACT172050.014.5162034
TATACAG137750.014.4088525
GTATTAT163700.014.09499551
GTATTGG158350.014.0315331
GTATTAG106350.014.0174651
TAGACAG79150.013.9181585
TACACTG107500.013.8696615
TTATACT172850.013.7622784
ACTGTGC108550.013.606418
ATAATAC156300.013.4879233
TAGACTG82600.013.4518035
GTATAGG103850.013.1205711
ATTATAC159300.013.1146873
CCTATAC102550.013.1030323
ACTATAC120350.012.94043353
ACTGTCC78550.012.9383998
TCTATAC114200.012.8889233
Produced by FastQC (version 0.11.2)