FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n02_49.35200000067dcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n02_49.35200000067dcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25649074
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG160000.016.6485845
GTATTAT179750.016.587121
TAATACT191850.015.9879064
CTATACT157400.015.29410654
TATACCG82850.015.130245
GTATATA336100.014.450651
GTATTAG116550.014.3794621
GTATAAG112850.014.3040021
TATACAG155550.014.2859885
TTATACT191650.014.1712474
GTATAGG120850.013.9463941
ATTATAC178500.013.9383783
ATAATAC177450.013.8067953
GTATAAT180100.013.6551451
TAGACTG87300.013.5975355
ATACTGT165950.013.5074866
GTATAGA134850.013.3434611
TCTATAC125450.013.2091233
GTATTGG173450.013.0016821
GTATTAC117550.012.8839281
Produced by FastQC (version 0.11.2)