FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n01_58.35100000068044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n01_58.35100000068044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397720
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT232800.019.4415534
TATACTG193750.019.0440925
CTATACT203600.018.6842544
GTATAGG165550.017.9931241
TTATACT245300.017.8119434
ATAATAC217350.017.7208143
TATACCG107900.017.6483445
GTATTAT216200.017.4035451
GTATTAG146800.017.2492161
GTATATA422700.017.2072371
TCTATAC160000.017.008153
GTATAAT225250.016.5566731
TATACAG182200.016.425
ATTATAC221100.016.3892173
TAGACTG106500.016.0967665
ACTGTGC144500.015.9711118
GTATAAG148400.015.9107511
CCTATAC163250.015.6222483
GTATAGA164550.014.8977281
GTATTAC148300.014.8322851
Produced by FastQC (version 0.11.2)