FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n01_57.35100000067fdb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n01_57.35100000067fdb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19206232
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC258770.13473230980444265No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG212950.11087547000369463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG134150.022.1594665
CTATACT154400.019.900414
TTATACT175950.019.7302744
TAATACT175400.018.9528054
GTATAGG113450.018.7177141
TAGACTG67750.017.9434645
ATAATAC148500.017.397173
CCTATAC115550.017.3850753
TATACAG133250.016.9991325
ATACTGT139200.016.613236
ATTATAC153650.016.5667923
ACTGTGC90650.016.553268
GTATTAT154600.016.4397071
GTATTGG135450.016.2036461
TATACTA219850.016.1350295
TCTATAC106150.015.8376033
TATACTT184100.015.7602485
GTATAAT162600.015.6600831
GTATAAG100550.015.5912751
TACACTG95450.015.5719875
Produced by FastQC (version 0.11.2)