FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n01_56.35100000067f61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n01_56.35100000067f61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15426126
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG107850.022.807465
CTATACT138850.020.5213094
CTGTGCG35650.018.2482539
TTATACT177950.018.173934
TAGACTG49800.018.0218685
ATACTGT108300.018.0207826
ACTGTGC78050.017.8868858
CTAGACT63450.017.5139434
TAATACT189150.017.1982364
TCTATAC108800.016.2813
TACACTG74100.016.2773175
ACTGTGT87600.016.2079228
TAACACT89650.015.7327944
CTACACT73200.015.7001564
GGACTGT41150.015.5784486
GTATAGA114700.015.5322261
CCTATAC93000.015.4214463
TACTGTG83550.015.4022127
GTATTGG101850.015.206261
TATACTC154550.015.1476485