FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n01_55.35100000067ee9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n01_55.35100000067ee9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22923986
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG138050.021.679841
TATACTG132300.019.3104975
CTATACT176850.019.0929684
CCTATAC144050.019.0886883
TATAGGG127950.017.259312
CCCTATA139300.016.091692
TATACTA233200.015.9646195
TTATACT169950.015.369174
TAATACT166900.015.2516694
TCTATAC109050.015.1552933
TATACCG76100.014.851265
GTATTAG100400.014.5736711
ATAATAC148100.014.55833153
ACTGTGC101600.014.5353468
GTATATA293900.014.4992271
TAGACTG77500.014.3378875
TATACAG135300.014.2494875
GTATTAT157350.014.0088731
TACACTG103250.013.9355335
GTATAGA110600.013.8309741
Produced by FastQC (version 0.11.2)