FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n01_51.35100000067e05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n01_51.35100000067e05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23864018
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG145200.016.5810955
ACTGTGC109650.015.3737478
TATACAG132800.015.0183795
TACACTG110850.014.9935415
TTATACT180000.014.8277954
GTATATA315750.014.8199831
GTATAAG99000.014.7798591
GTATTAT162050.014.7459831
CTATACT144500.014.6581724
GTATAGA124300.014.6380221
TCTATAC112100.014.5312243
GTATAGG101900.014.2193731
TAATACT182100.013.5353724
TAGACTG81650.013.43468955
GTATTAG108800.013.4049221
GTATAAT170900.013.2596221
GTATTGG151850.013.108531
GTGTAGA113750.013.0721711
ATAATAC162650.012.9349093
TAGACAG78750.012.8440175
Produced by FastQC (version 0.11.2)