FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l08n01_49.35100000067dc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l08n01_49.35100000067dc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25649074
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG160300.017.863135
TAATACT196550.016.0438214
CTATACT160550.015.9733574
TTATACT193350.015.7689814
GTATTAT183650.015.5989671
GTATATA335500.015.2932811
GTATAGG120500.014.7847381
ACTGTGC119250.014.7733288
ATAATAC179650.014.6452273
GTATAAT186100.014.6277551
GTATAGA132400.014.3888421
ATTATAC177250.014.2540743
GTATAAG120200.014.11021
TACACTG121800.013.8795865
CCTATAC119250.013.8590823
ATACTGT165150.013.8014756
TCTATAC128200.013.7806133
TATACCG87000.013.645665
TATACAG151100.013.6080925
ACTATAC131250.013.4965613
Produced by FastQC (version 0.11.2)