FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n02_47.35200000068126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n02_47.35200000068126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34241573
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT187850.016.7351194
TATACTG174300.016.1839925
GTATAGG144650.015.9876831
CCTATAC144250.014.9129563
TACACCG130900.014.2214225
TACACTG156700.014.1528685
TACACAG142950.014.0524745
GTATAGA158000.013.9155031
TATACAG174200.013.8760625
ACTGTGC152150.013.5795778
GTATATA358400.013.4749761
TAGACTG105950.013.4019155
GTGTAGG150400.013.2610061
TATACCG105450.013.2402855
TAATACT205000.013.0880994
GTATTAT189750.012.9384961
CTACACT150850.012.8438984
GTATTGG190000.012.7715121
TTATACT209400.012.6996974
CTACACC201100.012.6098864
Produced by FastQC (version 0.11.2)