FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n02_44.352000000680d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n02_44.352000000680d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21157271
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG127700.021.6055341
CTATACT157850.020.0059834
CCTATAC134550.019.5527063
TATAGGG120500.017.3793032
TATACTG120050.017.009855
TATACTA206900.016.9161365
TATACCG65750.016.5399385
CCCTATA125500.016.4598672
GTATATA257450.015.8076651
TAGACTG71450.015.68575
TATACAG122000.015.6480565
GTATTAT134300.015.5580961
TCTATAC97200.014.7055433
TAATACT152350.014.4634
GTATTGG126550.014.2969241
TTATACT154050.014.2417434
ATAATAC138050.014.1379433
GTATAAT144600.014.121471
ATACTAA160950.013.633166
GTATAAG90700.013.5603931
Produced by FastQC (version 0.11.2)