FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n02_41.3520000006806a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n02_41.3520000006806a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12130727
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC146220.1205368812602905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG84900.022.0944585
CTATACT89100.018.3874284
ACTGTGC58800.018.0938478
TTATACT124250.017.8866524
TAATACT121350.017.687984
GTATAGG58600.017.584541
ATACTGT84850.017.4085086
TAGGACT52950.017.2192764
GTATTAT104600.016.6156861
GTATTAG69050.016.5738051
TAGACTG42200.016.5424125
TACACTG62200.016.4913925
GTATAGA75900.016.2667371
ACTGTGT63600.016.2055158
TATACAG79300.015.8696245
ATAATAC104900.015.7083313
TAAGACT57400.015.636144
TATACCG41900.015.5274625
ATTATAC106550.015.5096443
TAGACAG43300.015.3544445
Produced by FastQC (version 0.11.2)