FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n02_40.35200000067fe5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n02_40.35200000067fe5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14426752
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG83450.020.259335
TAATACT101400.019.8569624
GTATAGG79900.019.6132261
CTATACT102150.019.1997954
GTATTAT97250.018.2626531
TTATACT108250.017.4598314
ATAATAC95450.017.3633843
TAGACAG49850.016.6714885
GTATATA181500.016.6141831
CCTATAC84750.016.4737513
GTATTGG96400.016.4039751
TCTATAC68050.016.0503543
TATACTA129100.015.9648535
ATTATAC92200.015.6061793
CTAATAC66400.015.5909773
ACTGTGC69100.015.4656868
TAGACTG48400.015.306675
TATAGGG77800.015.1986362
GTATAGA72450.015.1410321
GTATAAT99800.015.0837951
Produced by FastQC (version 0.11.2)