FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n02_39.35200000067f88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n02_39.35200000067f88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23379040
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT162100.021.473224
GTATAGG147300.020.8256971
TATACTG131150.020.0959855
TAATACT152950.018.9700324
CCTATAC149500.018.7724783
GTATAAG100900.017.2250181
TATACTA208500.016.9453875
TTATACT161500.016.9366024
TATAGGG132250.016.732612
ATTATAC143850.016.6708163
CCCTATA135650.016.5232582
GTATTAT150350.016.5184021
GTATATA275250.016.1824871
ATAATAC137650.015.9727653
ATACTGT138150.015.9519916
TAGACTG77750.015.81918055
TATACAG130750.015.6538165
ACTGTGT143450.015.2304748
CTAATAC94500.015.2260113
GTATAAT156800.015.2029331
Produced by FastQC (version 0.11.2)