FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n02_38.35200000067f2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n02_38.35200000067f2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23565754
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG144300.022.6735041
CTATACT174200.020.8265534
CCTATAC150150.020.5884743
TATAGGG134850.018.1705172
TAGACTG78250.017.9028765
TATACTG128450.017.5977845
CCCTATA141250.017.3472162
TATACTA218300.017.3376275
TACACCG81350.014.8856485
TACACTG109800.014.8778865
ACTGTGC110150.014.7901998
GTATATA277450.014.7190391
TCTATAC105250.014.6632343
TATACAG133600.014.4312745
GTATTGG147200.014.194191
TATACCG72450.014.1579525
GTATTAT140650.014.1124461
TAGGGAT154450.013.9277994
TAGACAG75650.013.8729375
ATACTGT137700.013.8659936
Produced by FastQC (version 0.11.2)