FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n02_35.35200000067ea3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n02_35.35200000067ea3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25096366
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG156200.022.77161
TATACTG150750.021.6425155
CTATACT190350.021.2062554
CCTATAC157450.019.544683
TAATACT186450.019.3829654
TTATACT195350.017.8678324
TATAGGG147950.017.8144872
ATTATAC172750.017.5387573
GTATTAT179100.017.4470921
TATACTA248300.017.443215
TCTATAC117950.017.1517283
TAGACTG85500.017.0522085
ATACTGT162100.016.935986
GTATATA329050.016.9144731
TATACAG155100.016.8100455
GTATAGA136200.016.73621
GTATTAG111250.016.4343871
GTATAAT175950.016.4099431
GTATAAG111650.016.3755071
GTATTAA151750.016.3668731
Produced by FastQC (version 0.11.2)