FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n01_47.35100000068129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n01_47.35100000068129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34241573
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG147750.015.7564211
TATACTG178550.015.6918125
TAGACTG106850.015.2884995
TATACAG175750.014.806975
GTATATA356150.014.7273681
CTATACT184450.014.7268674
TACACCG126750.014.1994715
CCTATAC143450.014.13598253
TACACTG156100.014.0242285
GTATTAG133000.013.9316321
GTATAGA148100.013.7943881
CTACACT155450.013.4111744
GTGTAGA163050.013.4037421
TATACTA243750.013.208885
TATACCG100900.013.1308855
GTGTAGG153150.013.1223731
TACACAG145500.012.8592065
ACTGTGC157850.012.8448028
ACAGTGC178850.012.8233758
GTATAAG128950.012.7848941
Produced by FastQC (version 0.11.2)