FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n01_44.351000000680d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n01_44.351000000680d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21157271
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG122550.021.903711
CTATACT165150.019.5537094
CCTATAC135000.019.0311183
TATACTG121350.018.4318375
TATAGGG117450.016.4567582
CCCTATA127550.016.270612
TTATACT157950.015.9651744
TACACTG96550.015.542555
TATACTA219100.015.4971265
TAGACAG70200.015.4235815
TCTATAC99000.015.3502563
ACTGTGC102900.015.2744378
TAATACT154000.015.2645234
GTATTAT142150.015.2405461
GTATAGA104350.014.933581
TATACAG125750.014.803545
GTATTGG123500.014.6953171
TAGACTG72700.014.6319135
GTATATA265050.014.4653271
ATAATAC141950.014.2185183
Produced by FastQC (version 0.11.2)