FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n01_41.3510000006806d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n01_41.3510000006806d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12130727
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC145380.1198444248230135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG82950.021.297625
TTATACT121750.019.0357384
CTATACT91050.018.8819774
TAATACT124550.018.684064
ATACTGT86900.018.1968756
TATACAG78900.017.8765625
TACACTG63050.017.7758945
GTATAGG60700.017.140831
TATACCG39150.017.1037735
ATAATAC104950.016.833833
TAAGACT54400.016.5872174
TAGACTG38100.016.453325
GTATTAT109600.016.1686861
ACTGTGC59250.016.0292788
ATTATAC104300.015.9369863
GTATAAG65200.015.8120651
TTACACT68300.015.7842534
TCTATAC72950.015.7547863
GTAATAC63250.015.6930813
TAGGACT53700.015.5652914
Produced by FastQC (version 0.11.2)