FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n01_40.35100000067fe8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n01_40.35100000067fe8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14426752
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG84800.019.3200535
CTATACT97900.019.2577254
CCTATAC83650.018.3941923
TAATACT107650.017.6899854
GTATAGG84300.017.5823231
TTATACT111900.017.2303094
ATAATAC97850.016.9866923
GTATAAT102500.016.5923671
GTATTAT98550.016.1968981
GTATTAG64850.016.1161731
GTATATA187950.015.7721711
TATACAG81350.015.6445235
TATACCG46150.015.3320295
ACTATAC69900.015.1505833
CTACACT69300.015.1445434
ACTGTGT86500.014.986398
TATACTA133050.014.9549255
TATACTC95950.014.7982835
ACTGTGC65350.014.60497958
GTATAGA69200.014.5538851
Produced by FastQC (version 0.11.2)