FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n01_39.35100000067f8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n01_39.35100000067f8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23379040
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT169950.020.9570484
GTATAGG144500.020.3501221
TATACTG132050.020.2104875
CCTATAC149200.018.2700043
TAGACTG75650.017.9529575
TAATACT158900.017.3935854
TATAGGG129300.017.2989162
TATACTA218900.016.942765
ATAATAC143600.016.6344763
GTATATA286200.016.482531
TTATACT173850.016.416854
GTATTAT152050.015.9653611
TATACAG131150.015.8231165
GTAATAC102100.015.8142573
GTATAAT158900.015.69566251
TCTATAC106300.015.6361693
ATACTGT136000.015.2924466
TAATACA142700.015.2083564
TATACCG77800.015.1984255
ATTATAC153500.015.1903833
Produced by FastQC (version 0.11.2)