FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n01_38.35100000067f2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n01_38.35100000067f2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23565754
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG142200.023.0533331
CCTATAC154850.020.2721273
CTATACT180250.020.1554494
TATAGGG134400.018.6217862
TATACTG132450.017.9989815
TAGACTG79750.017.5665935
CCCTATA144000.017.0835172
TATACTA230650.016.883275
TACACCG83450.015.6495745
TACACTG109100.015.4960645
TATACCG68850.015.1745195
ACTGTGC108750.014.9332498
TAGGGAT150850.014.8594594
GTATAGA114000.014.6697251
GTATTGG141600.014.5281011
TATACAG132700.014.3505865
GTATATA286500.014.2612651
GTGTAGG98350.014.2505571
TAATACT159600.014.2233314
CCTACAC103250.014.1667343
Produced by FastQC (version 0.11.2)