FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l07n01_35.35100000067ea6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l07n01_35.35100000067ea6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25096366
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG150800.021.6023775
GTATAGG152950.021.5263421
CTATACT196850.021.3017484
TAATACT187300.019.8017354
GTATTAT169650.019.1832941
CCTATAC163350.019.0423623
TTATACT198200.018.041864
TATACTA250100.017.8101635
ATTATAC174650.017.1305223
TATACCG80700.016.9471915
ATAATAC177300.016.8476963
GTATATA318850.016.7928331
TATAGGG141600.016.6012762
TATACAG152450.016.4157965
CCCTATA151200.016.2068042
GTATAAT180150.016.1663761
GTAATAC118350.015.9301883
ACTGTGC121300.015.8541078
TACACTG117950.015.8224355
GTATAAG117150.015.5325561
Produced by FastQC (version 0.11.2)