FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n02_33.35200000068140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n02_33.35200000068140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25399267
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG158300.023.0384481
CTATACT206350.022.989874
CCTATAC174350.020.754323
TATACTG154150.020.1173115
TAATACT198100.019.7043844
TATAGGG153950.018.8471072
TATACTA267850.017.5704275
TATACCG85200.017.502115
CCCTATA162300.017.4385722
GTATTAT183750.017.18571
GTATAAT181650.016.8615421
GTATATA342150.016.821281
TTATACT199300.016.5597214
TAGACTG87700.016.3533845
GTATAGA131650.015.7628051
ATAATAC176800.015.7394983
GTATTAG116300.015.679221
ATTATAC175400.015.6214643
TCTATAC123500.015.4957853
ATACTGT163750.015.3425876
Produced by FastQC (version 0.11.2)