FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n02_30.3520000006810c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n02_30.3520000006810c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24389712
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG162950.019.8176465
TAATACT257000.017.5166264
CTATACT191000.017.3290444
TTATACT244800.016.1199934
GTATAGG125600.015.879561
ATACTGT165850.015.5203296
TAGACTG78850.015.4785025
TACACTG116300.015.4759395
GGACTGT70500.015.0221776
GTATTAC127800.015.0116811
TATACAG151450.014.9570735
ACTGTGC116250.014.9112598
GTATTAT227350.014.7882621
CTAGACT96150.014.7671664
TAGACAG83700.014.7518135
GTATAAT227050.014.5777361
GTATTGG194500.014.5758381
TCTATAC150000.014.3727493
TGGACTG108300.014.2950825
TTACACT141650.014.2477944
Produced by FastQC (version 0.11.2)