FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n02_29.35200000068091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n02_29.35200000068091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18365297
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG131800.020.5733995
TTATACT205300.018.2962554
ACTGTGC84250.017.7561828
CTATACT151850.017.4499454
TAATACT214850.017.2619694
ATACTGT138100.016.8500796
TGTACTG136550.016.6930165
GTATTGG177000.016.6877461
CTGTGCG44250.016.635139
CTACACT89850.016.172534
TAGACTG57900.016.1574555
TAAGGCT75100.016.1240794
CTACCCT72000.015.7630424
CTGTGCA116650.015.6334299
TACACTG87300.015.6117995
GTATAGA132000.015.5054331
TAGACCT69950.015.410364
CTGTACT109750.015.3602584
TAGGACT77300.015.3580194
CTAGACT69450.015.1794274
Produced by FastQC (version 0.11.2)