FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n02_27.3520000006801a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n02_27.3520000006801a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20539139
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG141950.020.1734145
ACTGTGC92100.017.7902628
TAATACT241050.017.4741484
CTATACT168150.017.3965594
TTATACT233700.017.2515664
ATACTGT140650.016.8491256
TTACACT127700.015.5812634
TGTACTG148300.015.5629755
TACACTG98600.015.5569015
ACTGTGT115450.015.30283458
TCTATAC135300.015.0921173
TAAGGCT83650.014.9870964
GTATTAC113200.014.9763351
GTATAGG98650.014.970871
CTGTACT116600.014.9467594
CTGTGCA129950.014.7647779
TAAGACT99400.014.7144434
GTATTGG136250.014.7082051
TAGACTG67000.014.6012465
TAAGGGT73950.014.5769684
Produced by FastQC (version 0.11.2)