FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n02_26.35200000067fbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n02_26.35200000067fbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25540229
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT182250.019.594244
GTATAGG144800.019.3162271
CCTATAC154750.018.2584723
TATACCG79050.017.241745
TATACTG144950.016.9712035
TATACTA232450.015.9150865
TATAGGG142800.015.5633622
TAATACT179000.015.2543214
GTATATA298000.015.1863081
CCCTATA141850.015.0649932
TTATACT178050.014.4022364
TAGACTG83100.014.4012635
GTATAGA121200.014.3401611
GTATTAT168100.014.1246561
GTATTAG103600.014.0719461
ATAATAC163450.013.9164563
GTATAAT174000.013.754881
TATACAG138350.013.69603355
ATTATAC163250.013.642623
TCTATAC115650.013.5912843
Produced by FastQC (version 0.11.2)