FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n02_25.35200000067f1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n02_25.35200000067f1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17374798
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG99650.020.7293661
TATACTG115850.020.2911075
CTATACT144150.018.8432884
CCTATAC110300.018.7709523
TTATACT158800.018.6898084
TAATACT159450.018.4944924
GTATTAT144950.018.0184971
TATACCG56500.017.6509235
TAGACTG61450.017.3883045
ATTATAC135200.016.3675823
ACTGTGC83250.016.2586128
TATACAG106950.016.2072245
ATAATAC137050.016.0773413
TACACTG83050.015.7249635
GTATAAG88850.015.7131761
TATAGGG97350.015.51217652
CTACACT81950.015.4716844
TATACTA204250.015.4384115
GTATATA266100.015.2043831
GTAATAC85250.015.15129853
Produced by FastQC (version 0.11.2)