FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n01_33.35100000068143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n01_33.35100000068143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25399267
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG166050.021.9461171
CTATACT209050.020.7851944
CCTATAC178000.019.4223333
TATACTG163800.018.9017265
TAATACT198200.018.7364064
TATAGGG156750.018.1472592
TTATACT204900.016.964944
GTATTAT190000.016.95421
ATAATAC180050.016.6427943
TATACTA277900.016.6090775
GTATTAG116900.016.5823821
GTATATA347700.016.43851
CCCTATA162150.016.4007152
TATACCG84400.016.3726235
ATTATAC178000.016.3275663
GTATAAT182800.016.166481
GTATAAG118350.015.9376211
TCTATAC121200.015.6728293
GTATTGG157250.015.4696531
TATACAG155200.015.1454165
Produced by FastQC (version 0.11.2)