FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n01_30.3510000006810f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n01_30.3510000006810f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24389712
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG166350.020.4680335
TAATACT257500.017.6309074
CTATACT192500.017.4414544
TTATACT246250.017.4335634
ATACTGT167200.016.7847866
TAGACTG85950.016.2435595
ACTGTGC120100.015.8154518
TACACTG113650.015.58544355
GTATAGG123250.015.2654881
TTACACT144900.015.1414564
CTGTGCG57400.015.1394179
TAAGGCT99700.014.7182884
ATAATAC211100.014.4426243
GTATTGG189950.014.3573731
TATACAG148650.014.34369855
AATACTG143050.014.3076785
TATACCG85400.014.290755
GTATTAC124200.014.1923631
TCTATAC148900.014.1289013
TAGGACT106300.014.1171754
Produced by FastQC (version 0.11.2)