FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n01_29.35100000068094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n01_29.35100000068094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18365297
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG130450.020.0945785
TAGACTG60350.018.8063495
ATACTGT132750.018.5655256
TACACTG92700.018.0833475
TTATACT206400.017.8776574
TAATACT219300.017.843824
CTATACT146600.017.4284
ACTGTGC79750.016.7320848
TTACCCT79550.016.6557164
CTAGACT70600.016.4128654
AATACTG109400.016.3646955
TAGGACT79000.016.2907644
TAAGACT90100.016.2339884
TAAGGCT75350.016.1975424
TCTATAC122800.016.010543
GACTGTG50350.015.9390227
GTATTGG178650.015.9034511
CTACACT89600.015.9005634
CTACCCT73550.015.88374
CTGTGCG42200.015.5290399
Produced by FastQC (version 0.11.2)