FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n01_27.3510000006801d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n01_27.3510000006801d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20539139
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG139550.020.0775185
TAATACT242500.018.4475944
TAGACTG65250.017.3214535
CTATACT167250.017.1502844
TTATACT225800.016.7833844
ACTGTGC91850.016.647658
ATACTGT142300.016.5187266
TATACAG129150.015.9581735
TAAGACT99950.015.8220224
CTACACT100050.015.5688774
ACTGTGT118400.015.2408128
GTATAAG115050.015.1963171
TCTATAC136800.014.892753
TACTGTG109400.014.8885717
TAAGGCT84600.014.8756544
CTGTGCG47000.014.8525139
TAGGACT86400.014.840574
TACACTG94650.014.800945
AGTACTG115150.014.7228585
CTAGACT90600.014.5719324
Produced by FastQC (version 0.11.2)