FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n01_26.35100000067fb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n01_26.35100000067fb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25540229
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG147250.020.1983721
CCTATAC158150.018.7974223
CTATACT187500.018.615444
TATACTG141550.017.4784625
TATACTA244100.016.3218565
TATAGGG138150.015.8812682
CCCTATA148200.015.7335722
TAGACTG84700.015.5300095
GTATTAT171200.015.0138061
GTATATA305000.014.954391
TAATACT186650.014.68030554
TTATACT180500.014.62799554
TATACCG83450.014.3969175
ATAATAC167000.014.132963
TATACAG140450.013.6256955
ACTGTGC115950.013.3508668
GTATTGG156600.013.0762391
TCTATAC116650.012.9867113
ATTATAC160150.012.9582913
GTATTAA153600.012.929521
Produced by FastQC (version 0.11.2)