FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n01_25.35100000067f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n01_25.35100000067f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17374798
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG110450.022.0558835
GTATAGG101500.021.251041
CTATACT146450.020.4284444
TTATACT158300.019.469194
TAATACT156650.018.8254454
CCTATAC109800.018.1217773
GTATTAT146550.017.7658121
ATAATAC136300.017.7341773
ATTATAC136850.016.7953723
ATACTGT113900.016.5094186
TCTATAC94050.016.258713
TATAGGG100550.016.246712
TATACAG106250.015.910875
GTATTAG87350.015.8821871
ACTGTGC89100.015.6685928
TAGACTG61750.015.6110435
TATACTA205350.015.5861215
GTATATA264600.015.2443251
TATACTT161500.015.084045
GTATTAC92000.014.9761621
Produced by FastQC (version 0.11.2)