FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l06n01_24.35100000067e99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l06n01_24.35100000067e99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28044336
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG163800.019.6952691
CTATACT204650.019.6313464
TATACTG166050.019.5327435
CCTATAC166800.018.0505713
TAGACTG94400.016.9024125
TATACAG163300.016.6628725
GTATATA357400.016.4046381
TTATACT211350.016.335174
TATAGGG160650.015.9333282
TAATACT201950.015.8492074
GTATTAT192850.015.8415391
TATACTA274850.015.636315
ATAATAC189500.014.9109543
TCTATAC132100.014.8112633
ATTATAC190850.014.7557143
TATACCG89300.014.7302375
ACTGTGC129050.014.7186488
GTATTAG122300.014.4906671
TACACTG126150.014.2670235
GTATAAT198200.014.2632771
Produced by FastQC (version 0.11.2)