FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n02_23.352000000680e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n02_23.352000000680e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24799005
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG129550.019.4661461
TATACTG133750.018.3570945
CTATACT156000.016.6824074
GTATATA296650.015.5772721
CCTATAC126150.015.547463
TATACCG80200.015.2182925
TTATACT171200.015.173524
GTATAAG103150.015.0096751
TAGACTG81300.014.9539725
TAATACT170150.014.84849554
TATACAG140250.014.4926595
TATACTA213200.014.4788245
GTATTAT164000.014.1897631
ATTATAC161250.014.1069263
GTATAGT129150.013.9369491
GTATTGG160150.013.8488231
ATAATAC157050.013.8189453
GTATAGA120400.013.8058921
TACACCG85000.013.632585
GTATAAT162850.013.5900491
Produced by FastQC (version 0.11.2)