FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n02_18.35200000068084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n02_18.35200000068084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19346989
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG119600.019.2582955
CTATACT129300.018.1072884
GTATAGG90300.017.6721841
GTATTGG176750.016.7673281
GTATATA250950.016.6356721
TAATACT146300.016.3927614
CCTATAC89600.016.2717913
TTATACT148800.016.1492584
GTATTAT138950.015.4496471
TATACAG115500.015.3778945
ACTGTGC88600.015.0629938
GTATAAG85350.014.9688161
ATAATAC133850.014.9017033
GTATAGA102400.014.8882541
GTATTAG86700.014.7905171
TATACCG59850.014.6796215
TATTGGG183400.014.6315392
GTATAAT138500.014.2310511
TAGACAG66400.013.94677455
TATACTA178050.013.8964225
Produced by FastQC (version 0.11.2)